Cloning and Expression Analysis of Vv-lac3, A Novel Functional Laccase Gene Involved in Stipe Elongation, Based on the Genomic Sequence of Volvariella volvacea
نویسندگان
چکیده
Laccases (benzenediol: oxygen oxidoreductases, EC 1.10.3.2) belong to a family of blue multicopper oxidases that catalyze the oxidation of a variety of aromatic substrates along with the reduction of molecular oxygen to water [1]. Laccases have attracted extensive attention due to their functional diversity; for example, they are involved in lignin biodegradation, plant pathogenesis, and stress defense [2]. The multigene family of laccases is a common feature in fungi [1]. The first example of the multigene family of laccases was described in Agaricus bisporus [3]. Since, seventeen laccases have been characterized from Coprinopsis cinerea and, eleven laccases from Laccaria bicolor [4] and Flammulina velutipes have been characterized [5].
منابع مشابه
Cloning and Expression Analysis of Vvlcc3, a Novel and Functional Laccase Gene Possibly Involved in Stipe Elongation
Volvariella volvacea, usually harvested in its egg stage, is one of the most popular mushrooms in Asia. The rapid transition from the egg stage to elongation stage, during which the stipe stretches to almost full length leads to the opening of the cap and rupture of the universal veil, and is considered to be one of the main factors that negatively impacts the yield and value of V. volvacea. St...
متن کاملGene Expression Profiling Reveals Large Regulatory Switches between Succeeding Stipe Stages in Volvariella volvacea
The edible mushroom Volvariella volvacea is an important crop in Southeast Asia and is predominantly harvested in the egg stage. One of the main factors that negatively affect its yield and value is the rapid transition from the egg to the elongation stage, which has a decreased commodity value and shelf life. To improve our understanding of the changes during stipe development and the transiti...
متن کاملA specific type of cyclin-like F-box domain gene is involved in the cryogenic autolysis of Volvariella volvacea.
Cryogenic autolysis is a typical phenomenon of abnormal metabolism in Volvariella volvacea. Recent studies have identified 20 significantly up-regulated genes via high-throughput sequencing of the mRNAs expressed in the mycelia of V. volvacea after cold exposure. Among these significantly up-regulated genes, 15 annotated genes were used for functional annotation cluster analysis. Our results sh...
متن کاملThe Sequence Characteristics and Expression Models Reveal Superoxide Dismutase Involved in Cold Response and Fruiting Body Development in Volvariella volvacea
As the first defence for cells to counteract the toxicity of active oxygen, superoxide dismutase (SOD) plays an important role in the response of living organisms to stress and cell differentiation. One extracellular Cu-ZnSOD (ecCu-ZnSOD), and two MnSODs, were identified based on the Volvariella volvacea genome sequence. All three genes have complicated alternative splicing modes during transcr...
متن کاملInduction of laccase activity in the edible straw mushroom, Volvariella volvacea.
Volvariella volvacea, strain V14, produces multiple forms of extracellular laccase when grown in submerged culture in a defined medium with glucose as sole carbon source, and on cotton waste 'compost' representative of the conditions used for industrial-scale mushroom cultivation. In liquid culture, enzyme synthesis is associated with the onset of secondary growth, and is positively regulated b...
متن کامل